Protein production in filamentous fungal cells in the absence of inducing substrates

US10876103B2 · kind B2 · utility

Assignees

• Danisco US Inc. · US
• VTT Technical Research Centre of Finland Ltd · FI

Inventors

• Michael Ward · San Francisco, US
• Yun Luo · Santa Rosa, US
• Felipe Oseas Bendezu · Knowles Crossroads, US
• Mari Valkonen · Tuusula, FI
• Markku Saloheimo · Helsinki, FI
• Nina Aro · Espoo, FI
• Tiina Pakula · Kihniö, FI

Key dates

• Filing date: Oct 3, 2017
• Grant date: Dec 29, 2020
• Expiry date: Oct 3, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01004
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.