Method for determining the overall brightness of at least one object in a digital image

US10878562B2 · kind B2 · utility

Assignee

• Qiagen GmgH · DE

Inventors

• Maiko Lohel · Hilden, DE
• Björn Labitzke · Cologne, DE
• Thorsten Zerfass · Nuremberg, DE

Key dates

• Filing date: Dec 9, 2016
• Grant date: Dec 29, 2020
• Expiry date: Jan 22, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30072
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a method and a system for the overall brightness of at least one object in a digital image. In particular, the method for determining the overall brightness of at least one object in a digital image, the at least one object being configured to receive molecules comprising genetic information, the method comprising the steps of: generating an area A comprising k sample points, the k points being distributed in the area A according to a predetermined distribution; aligning the center position of the area A to the center position of the at least one object in the digital image; extracting the intensity value at each of the k sample points; and calculating the central tendency of the extracted intensity values at the respective k sample points.