DNA polymerase variant and application thereof

US10883091B2 · kind B2 · utility

Assignee

• MGI Tech Co., Ltd. · CN

Inventors

• Lin Wang · University City, US
• Fen Liu · 流花街道, CN
• Yuliang Dong · Longbeilingcun, CN
• Wenwei Zhang · Longbeilingcun, CN
• Chongjun Xu · San Jose, US
• Snezana Drmanac · Nanhu, CN

Key dates

• Filing date: Jul 8, 2019
• Grant date: Jan 5, 2021
• Expiry date: Jul 8, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/43
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed in the present disclosure is a recombinant DNA polymerase. The recombinant DNA polymerase is any one selected from: A) a protein, having amino acid modifications at positions 408, 409 and 485, and at least one of amino acid modification(s) at positions 53, 59, 199, 243, 526, 558, 613, 641, 671, 673, 674, 692 and 709 compared to the amino acid sequence of a wild-type KOD DNA polymerase; B) a protein derived from the protein in A), formed by deleting amino acids 1 to 29 from a C-terminus of the protein in A) and keeping the remaining amino acids unchanged; and C) a protein derived from the protein in A) or B), formed by connecting a tag to the N-terminus or C-terminus of the amino acid sequence of the protein in A) or B), wherein the protein in A), B) and C) has DNA polymerase activity.