Method to detect activity of a polymerase

US10883137B2 · kind B2 · utility

Assignees

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US
• Lead Discovery Center GmbH · DE

Inventors

• Tim Bergbrede · Dortmund, DE
• Nils-Göran Larsson · Huddinge, SE
• Claes Gustafsson · Belmont, US
• Maria Falkenberg-Gustafsson · Mölndal, SE

Key dates

• Filing date: May 30, 2016
• Grant date: Jan 5, 2021
• Expiry date: Apr 1, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/91245
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as forster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.