Method for preparing nanocomposite and label-free aptamer electrochemical sensor of gamma-interferon based on the nanocomposite
US10883984B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 21, 2019 |
| Grant date | Jan 5, 2021 |
| Priority date | — |
| Expiry date | Mar 21, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/57
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a label-free aptamer electrochemical sensor of γ-interferon based on a dendrimer/gold nanoparticle/molybdenum disulfide nanocomposite is provided. The nanocomposite is drip-coated on a surface of an electrode to prepare a modified electrode, and a terminal sulfhydryl group of a γ-interferon aptamer chain is connected to the gold nanoparticle via Au—S bond to obtain a nanocomposite-aptamer modified electrode. When γ-interferon is present, the γ-interferon specifically binds to the aptamer chain on the sensor, resulting in the aptamer's hairpin structure being opened and stretched, which can effectively adsorb methylene blue MB in the electrolyte, causing significant enhancement of MB redox signal. A linear relationship between a current intensity of MB oxidation peak and a concentration of the γ-interferon is fitted to construct the label-free aptamer electrochemical sensor of γ-interferon.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.