Mutated immunoglobulin-binding polypeptides

US10889615B2 · kind B2 · utility

Assignee

• Cytiva BioProcess R&D AB · SE

Inventors

• Gustav Rodrigo · Uppsala, SE
• Tomas Bjorkman · Uppsala, SE
• Mats Ander · Uppsala, SE

Key dates

• Filing date: May 10, 2017
• Grant date: Jan 12, 2021
• Expiry date: May 10, 2037

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/52
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO: 4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.