Method for genetic transformation of edible mushrooms

US10889824B2 · kind B2 · utility

Assignee

• Shanghai Academy of Agricultural Sciences · CN

Inventors

• Jianyu LIU · Chengdu, CN
• Xiaodong Shang · Shanghai, CN
• Chunyan Song · Shanghai, CN
• Qi Tan · Shanghai, CN
• Zhen-Guang Xu · 魏塘街道, CN
• Qiaozhen Li · Shanghai, CN
• Dan Zhang · San Diego, US
• Ruijuan Wang · Shanghai, CN
• Hailong Yu · Beijing, CN
• Lujun ZHANG · Shanghai, CN
• Meiyan Zhang · Nanhu, CN
• Hui Yang · Shanghai, CN
• Yu Li · Shanghai, CN
• Feng Zhou · Shanghai, CN
• Ning Jiang · 红钢城街道, CN

Key dates

• Filing date: Jul 17, 2018
• Grant date: Jan 12, 2021
• Expiry date: Jan 30, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/87
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for genetic transformation of edible mushrooms is provided relating to the technical field of genetic transformation of Agaricus bisporus, enoki mushroom and shiitake. The disclosed method for genetic transformation of Agaricus bisporus includes: inoculating Agaricus bisporus liquid mycelia into a foxtail-millet-grain culture medium, and pre-culturing them at 20-25° C. until Agaricus bisporus mycelia grow on surfaces of foxtail millet grains. This method uses the foxtail millet grains as an attachment matrix, and during the pre-culturing and co-culturing, the culture substrate is shaken up every day. The method for genetic transformation of enoki mushroom or shiitake includes: inoculation of enoki mushroom mycelia or shiitake mycelia, activated culturing of agrobacterium, and agrobacterium infecting a foxtail millet grain-enoki mushroom mycelium matrix or a foxtail millet grain-shiitake mycelium matrix.