Patent · US Active

Image differentiated multiplex assays for multiplex detection of DNA mutations

US10894975B2 · kind B2 · utility

2Cited by
15References
17Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 11, 2017
Grant dateJan 19, 2021
Priority date—
Expiry dateAug 1, 2038

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2021/6439
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Provided herein are methods and kits for detecting the presence of DNA mutations in the KRAS, BRAF, CTNNB1, and APC genes. The methods and kits employ microcarriers, each with a probe specific for a DNA mutation in the KRAS, BRAF, CTNNB1, or APC gene and an identifier unique to the probe sequence. Upon isolation and amplification of DNA from a sample, hybridization of amplified DNA with a probe, specific for a DNA mutation, that is coupled to a microcarrier indicates the presence of the DNA mutation in the sample. Since each microcarrier can be identified through detection of the identifier, multiplex screening assays for multiple mutations in each of the KRAS, BRAF, CTNNB1, and APC genes are provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.