Patent · US Active

Compositions, methods, and kits for isolating nucleic acids

US10894976B2 · kind B2 · utility

51Cited by

65References

17Claims

0Family size

Assignee

Natera, Inc. · US

Inventors

James Stray · San Mateo, US
Jason Tong · San Carlos, US
Xiaoke Wang · Zhejiang, CN

Key dates

Filing date	Feb 15, 2018
Grant date	Jan 19, 2021
Priority date	—
Expiry date	Feb 15, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/1006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed here is a composition for isolating nucleic acids from a biological sample, comprising a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof. Also disclosed is a method for binding nucleic acids to a matrix, comprising: contacting the nucleic acids from a biological sample with the matrix in the presence of a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof, thereby binding the nucleic acids to the matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample comprising a binding buffer, wherein the binding buffer comprises a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.