Method for preparing ratiometric electrochemical aptasensor for Vaniline based on nanocomposite modified electrode
US10895552B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 2019 |
| Grant date | Jan 19, 2021 |
| Priority date | — |
| Expiry date | Mar 14, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5438
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a ratiometric electrochemical aptasensor for vanillin based on an aptamer-gold nanoparticle-ferrocene-Ketjenblack-ZIF-8 nanocomposite modified electrode includes, dripping ZIF-8 nanocomposite doped with ferrocene and Ketjenblack on the surface of the bare glassy carbon electrode, and immersing this modified electrode in the chloroauric acid solution. The cyclic voltammetry is employed to scan and electrodeposit gold nanoparticles to obtain the gold nanoparticle-deposited ZIF-8 nanocomposite. The aptamer of vanillin is attached to gold nanoparticles via Au—S bonds to construct an aptamer-coupled nanocomposite sensing platform. Then, the electrochemical curves in the presence of different vanillin concentrations are measured, the linear relationship between the ratios of the current peak intensities of ferrocene and vanillin and the molar concentrations of vanillin is fitted, and the ratiometric electrochemical aptasensor for vanillin is constructed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.