Methods for generating engineered enzymes
US10900059B2 · kind B2 · utility
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32Claims
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Key dates
| Filing date | Oct 14, 2015 |
| Grant date | Jan 26, 2021 |
| Priority date | — |
| Expiry date | Jan 3, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96466
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., “cleaveOme”) inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.
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