High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
US10900073B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 2016 |
| Grant date | Jan 26, 2021 |
| Priority date | — |
| Expiry date | Dec 9, 2036 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/165
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.