Systems and methods for nucleic acid sequencing

US10900075B2 · kind B2 · utility

Assignee

• GENAPSYS, INC. · US

Inventors

• Hesaam Esfandyarpour · Redwood City, US
• Maryam Jouzi · Redwood City, US
• Seth Stern · Palo Alto, US
• Paul Kenney · Sunnyvale, US

Key dates

• Filing date: Sep 25, 2018
• Grant date: Jan 26, 2021
• Expiry date: Sep 25, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides methods and systems for sequencing nucleic acid molecules. Methods may include sequencing double-stranded nucleic acids or single-stranded nucleic acids. Sequencing may include the use of nucleotides coupled to electrostatic moieties. The electrostatic moieties may be detected by a sensor array. The electrostatic moieties may be reversible electrostatic moieties that are cleaved from the nucleic acid molecule after incorporation of the nucleotide. The electrostatic moieties may be irreversible electrostatic moieties. Nucleotides comprising irreversible electrostatic moieties may be incorporated into the nucleic acid molecule, detected by the sensor array, and exchanged for non-detectable nucleotides.