Coupled peptide chain for dissolving poorly soluble polypeptides and application thereof for separation and purification in liquid chromatography
US10906933B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 2016 |
| Grant date | Feb 2, 2021 |
| Priority date | — |
| Expiry date | Mar 6, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.