Modified guide RNAs, CRISPR-ribonucleotprotein complexes and methods of use
US10907150B2 · kind B2 · utility
1Cited by
5References
37Claims
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Key dates
| Filing date | Jun 14, 2018 |
| Grant date | Feb 2, 2021 |
| Priority date | — |
| Expiry date | Jun 14, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are modified guide RNAs such as a single guide RNA including, from 5′ to 3′, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.
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