Method for isolating cross-reactive aptamer and use thereof
US10907162B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 14, 2020 |
| Grant date | Feb 2, 2021 |
| Priority date | — |
| Expiry date | Feb 14, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/31
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.