Double-strand DNA break quantification method
US10907199B2 · kind B2 · utility
0Cited by
0References
19Claims
0Family size
Inventors
Key dates
| Filing date | Oct 23, 2018 |
| Grant date | Feb 2, 2021 |
| Priority date | — |
| Expiry date | Oct 23, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y605/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides the quantification of double-strand breaks in DNA molecules using terminal deoxynucleotidyl transferase using a preliminary step of nick gap and repair. This preliminary step comprising contacting the DNA molecules with both a DNA ligase and a DNA polymerase to repair DNA nicks and remove DNA gaps prior to using the terminal deoxynucleotidyl transferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.