Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
US10907206B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Oct 24, 2019 |
| Grant date | Feb 2, 2021 |
| Priority date | — |
| Expiry date | Oct 24, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Aspects of the invention relate to methods for preparing and analyzing a sequencing library from a mixed cell-free DNA (cfDNA) sample, wherein the mixed sample includes double-stranded DNA (dsDNA), damaged dsDNA (e.g., nicked dsDNA), and single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared from dsDNA alone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.