Method for determining whether a peptide comprises aspartate or isoaspartate

US10908165B2 · kind B2 · utility

Assignees

• Purdue Research Foundation · US
• AMGEN INC. · US

Inventors

• Stephen Ayrton · West Lafayette, US
• Robert Graham Cooks · West Lafayette, US
• Tawnya Flick · Thousand Oaks, US
• Da Ren · Thousand Oaks, US

Key dates

• Filing date: Jun 7, 2018
• Grant date: Feb 2, 2021
• Expiry date: Dec 6, 2038

Classification

• Technology area (CPC H): Electricity
• CPC primary: H01J49/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.