Method for preparing ratiometric fluorescent probe for melamine based on silver nanocluster complex
US10913892B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 5, 2019 |
| Grant date | Feb 9, 2021 |
| Priority date | — |
| Expiry date | Mar 5, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6432
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a ratiometric fluorescent probe for melamine based on a DNA-stable silver nanocluster-rhodamine 6G complex, wherein the electrostatic self-assembly technology is adopted to construct a silver nanocluster-rhodamine 6G complex. The melamine forms a strong hydrogen bond with thymine in the DNA of the surface of the silver nanocluster, causing rhodamine 6G to dissociate from the surface of the silver nanocluster, destroying the fluorescence resonance energy transfer, so as to restore the fluorescence of the silver nanocluster. This process has little effect on Rhodamine 6G fluorescence which can be used as a reference signal, while silver nanocluster fluorescence can be used as a response signal. By fitting the linear relationship between the ratio of fluorescence emission peak intensities of the silver nanocluster to rhodamine 6G and the molar concentration of the melamine, the ratiometric fluorescent probe for melamine can be constructed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.