Three-channel fluorescence localization super-resolution biological microscope system and method
US10914680B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 3, 2020 |
| Grant date | Feb 9, 2021 |
| Priority date | — |
| Expiry date | Jul 3, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0633
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A three-channel fluorescence localization super-resolution biological microscope system includes a microscope main body, an illumination module (2), an imaging module (3) and a three-dimensional nanoscale sample locking module (4). The illumination module (2) is used to realize three channels, wide-field and semi-total internal reflection illumination and total internal reflection illumination and is adjustable in an illumination mode. Illumination light generated by the illumination module (2) illuminates a sample through an objective lens. Fluorescent light emitted from the sample is allocated by a light splitter (8) of a fixed ratio after passing through a first multicolor reflector (6) and a front imaging lens (7), one part is allocated into the three-dimensional nanoscale sample locking module (4), and the remaining part is allocated into the imaging module. A three-channel fluorescence localization super-resolution biological microscope method is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.