Patent · US Active

Stimulated emission depletion nonlinear structured illumination microscopy (STED-NSIM) apparatus, methods, and applications

US10914930B2 · kind B2 · utility

3Cited by

0References

17Claims

0Family size

Assignee

Arizona Board of Regents on Behalf of the University of Arizona · US

Inventors

Leilei Peng · Tucson, US
Yu Li · Hong Kong, CN
Han Zhang · Nanhu, CN

Key dates

Filing date	Aug 30, 2017
Grant date	Feb 9, 2021
Priority date	—
Expiry date	Aug 30, 2037

Classification

Technology area (CPC G)Physics
CPC primaryG02B27/58
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A superresolution STED-NSIM apparatus having an epifluorescence architecture utilizing a 2D structured STED pattern having a N.A. less than a N.A. of the microscope objective and no surface plasmon resonance (SPR) effects. A superresolution STED-NSIM imaging method using a fully deterministic imaging processing method, in which a pre-calibrated set of parameters are used to process all image data.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.