Thermolabile Serratia Marcescens nuclease
US10920202B1 · kind B1 · utility
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Key dates
| Filing date | Aug 20, 2020 |
| Grant date | Feb 16, 2021 |
| Priority date | — |
| Expiry date | Aug 20, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/30002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is an engineered thermolabile mutant of Serratia Marcescens Nuclease. In the same buffer as used for optimal enzyme activity, after 60° C. for 20 min, while the wild type retained 12.5% activity, the thermolabile mutant R146D/D156R/D229R/D245R (SEQ ID NO: 12, without the first 21 amino acids, which are a signal peptide) retained only 0.39% activity. Heat inactivation of the mutant, when it is used for protein purification, can be used after a period when substantial DNA in a protein has been degraded, but before unwanted degradation takes place.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.