Detection of particle-contained reverse transcriptase activity
US10920288B2 · kind B2 · utility
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1References
19Claims
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Assignee
Inventors
Key dates
| Filing date | Jan 15, 2016 |
| Grant date | Feb 16, 2021 |
| Priority date | — |
| Expiry date | Jan 15, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/10023
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods and kits for detecting in a sample the presence of a virus particle or a virus-like particle that has reverse transcriptase activity and methods for preparing a retroviral contaminant-free substance. An aspect of the present invention is a method for detecting the presence of a virus particle in a sample of a Virus-like Particle (VLP) drug substance comprising a step of performing PCR-based reverse transcriptase (PBRT) on a sample of the VLP drug substance that has been treated with a protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.