Probes for quantitative imaging of thiols in various environments
US10921327B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 10, 2015 |
| Grant date | Feb 16, 2021 |
| Priority date | — |
| Expiry date | Aug 1, 2036 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/182
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Embodiments of the present disclosure pertain to methods of detecting a thiol in an environment by exposing the environment to a probe molecule that contains a marker and a thiol responsive group. The thiol responsive group reversibly reacts with the thiol in the environment to form a probe-thiol adduct. This in turn causes a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct, which can then be correlated to the presence of the thiol in the environment. The correlation can occur by quantifying the thiol concentration in the environment. In addition, thiol detection can occur in real-time. Further embodiments of the present disclosure pertain to probe molecules that are utilized for detecting a thiol in an environment. In some embodiments, the probe molecule includes a marker and a thiol responsive group. In some embodiments, the probe molecule also includes an organelle targeting moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.