Patent · US Active

Method of creating characteristic peak profiles of mass spectra and identification model for analyzing and identifying microorganizm

US10930371B2 · kind B2 · utility

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Key dates

Filing dateJul 10, 2017
Grant dateFeb 23, 2021
Priority date
Expiry dateDec 16, 2039

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG06N20/00
  • WIPO fieldComputer technology
  • WIPO sectorElectrical engineering

Abstract

A method of creating characteristic peak profiles of mass spectra and identification model for analyzing and identifying microorganisms are provided. MALDI-TOF MS data of microorganisms having the same feature are gathered. Discretization of the data is performed. Density-based clustering is used to find m/z values of spectral peaks with high probability of occurrence from the discretized data. A characteristic MS peak profile is created for every specific feature of microorganisms. Every such a characteristic profile forms a feature template. The mass spectrum of each known isolate is matched against all the feature templates and a number of matched vectors are obtained. The matched vectors are then concatenated into a single “integrated vector.” Then, a machine learning method and the integrated vectors generated from all known isolates are used to create a classification model for microorganism identification.

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