Separation of VWF and VWF propeptide by chromatographic methods
US10934340B2 · kind B2 · utility
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18References
28Claims
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Key dates
| Filing date | Mar 20, 2019 |
| Grant date | Mar 2, 2021 |
| Priority date | — |
| Expiry date | Mar 20, 2039 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to a method for separating a mature von Willebrand Factor (mat-VWF) from von Willebrand Factor pro-peptide (VWF-PP) by incubating a composition comprising inducing dissociation of mat-VWF and VWF-PP by disruption of the non-covalently associated mat-VWF and VWF-PP, wherein said dissociation is induced by: (i) addition of at least one chelating agent, or (ii) increasing the pH to a pH of at least 7, and then collecting said mat-VWF to obtain a high purity, propeptide depleted mature VWF (mat-VWF).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.