Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10934584B2 · kind B2 · utility
0Cited by
8References
19Claims
0Family size
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Key dates
| Filing date | Apr 23, 2018 |
| Grant date | Mar 2, 2021 |
| Priority date | — |
| Expiry date | Aug 10, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B80/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.