Detection and analysis method for urine-modified nucleoside based on surface-enhanced resonance Raman spectroscopy
US10935495B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 18, 2016 |
| Grant date | Mar 2, 2021 |
| Priority date | — |
| Expiry date | Aug 30, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/493
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses a detection and analysis method for urine-modified nucleoside based on a surface-enhanced resonance Raman spectroscopy technology. In the method, a tumor marker modified nucleoside in the urine of a normal person and a cancer patient is extracted through the specificity of borophenylic acid gel; Au colloid is taken as an enhancing substrate to detect SERRS signals; and statistical analysis is conducted by using PLS-DA algorithms to establish a diagnosis and identification model for the SERRS of the urine-modified nucleoside. The model is used to discriminate that the to-be-detected urine-modified nucleoside belongs to the normal person or the cancer patient. After PLS-DA, the surface-enhanced resonance Raman spectroscopy data of the urine-modified nucleoside of the present invention has specificity of 96.9%, sensitivity of 98.2% and accuracy of 97.6%. The present invention has the characteristic of rapid and objective detection, and can provide important reference for doctors to diagnose esophagus cancer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.