Device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation

US10940476B2 · kind B2 · utility

Assignee

• ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY · US

Inventors

• Laimonas Kelbauskas · Gilbert, US
• Honor Glenn · Mesa, US
• Jeff Houkal · Los Angeles, US
• Clifford L. Anderson · Tempe, US
• Yanqing Tian · Tempe, US
• Fengyu Su · Tempe, US
• Deirdre Meldrum · Phoenix, US

Key dates

• Filing date: Apr 21, 2017
• Grant date: Mar 9, 2021
• Expiry date: Nov 20, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/48735
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.