Strain of bacteria producing DHA and EPA, six gene fragments in the bacterial genome and their applications

US10941185B2 · kind B2 · utility

Assignee

• XIAMEN HUISON BROTECH CO., LTD · CN

Inventors

• Liyi Chen · Tokyo, JP
• Huichang Zhong · Wuxi, CN
• Shuirong CHEN · Xiamen, CN

Key dates

• Filing date: Nov 26, 2019
• Grant date: Mar 9, 2021
• Expiry date: Nov 26, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/89
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved. The bacteria provided by the invention, the six gene fragments, the protein encoded by these six gene fragments, the vector, the cell or the organism containing these six gene fragments all have important application values.