Vinylisomerase-dehydratases, alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases and methods for making and using them

US10941454B2 · kind B2 · utility

Assignees

• Genomatica, Inc. · US
• Braskem S.A. · BR

Inventors

• Stephanie J. Culler · San Diego, US
• Robert Haselbeck · San Diego, US
• Harish Nagarajan · San Diego, US
• Iuri Estrada Gouvea · Campinas, BR
• Daniel Koch · Shanghai, CN
• Mateus Schreiner Lopes · Camaçari, BR
• Lucas Pedersen Parizzi · Camaçari, BR

Key dates

• Filing date: May 26, 2016
• Grant date: Mar 9, 2021
• Expiry date: May 26, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.