Method for measuring concentration of test substance, and detection apparatus
US10942127B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 2014 |
| Grant date | Mar 9, 2021 |
| Priority date | — |
| Expiry date | Jul 30, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/904
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provide a method for detecting a test substance utilizing a color development caused by an enzyme reaction, or a color development caused by a specific interaction such as an antigen-antibody reaction and an enzyme reaction, the test substance being detected rapidly, sensitively and quantitatively without using a spectroscopic measurement device. The method for measuring a concentration of a test substance by the present invention comprises the steps of: generating a peroxide from the test substance; obtaining a polymerized substance by bringing an oxidoreductase for producing a polymerized substance and a substrate of the oxidoreductase for producing a polymerized substance into contact with the peroxide; and irradiating the polymerized substance with light to record a temporal variation information of an intensity of scattered light generated from an irradiation point.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.