ARRDC1-mediated microvesicles (ARMMS) and uses thereof

US10945954B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Quan Lu · Shanghai, CN
• Joseph F. Nabhan · Cambridge, US
• Stanley N. Cohen · Stanford, US

Key dates

• Filing date: Aug 4, 2017
• Grant date: Mar 16, 2021
• Expiry date: Mar 29, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/726
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provide isolated arrestin domain-containing protein 1 (ARRDC1)-mediated microvesicles (ARMMs). Methods for generating and for isolating ARMMs are also provided herein. ARMMs can be used to deliver agents, for example, nucleic acids (e.g., siRNAs, microRNAs, lincRNAs), proteins (e.g., transcription factors, chromatin modulators, kinases, phosphorylases, or recombinases), or small molecules to target cells in vitro and in vivo, and methods for such ARMM-mediated delivery are provided herein. Diagnostic and therapeutic methods using ARMMs are also described herein.