Methods for directed differentiation of pluripotent stem cells to immune cells

US10947502B2 · kind B2 · utility

Assignee

• FUJIFILM Cellular Dynamics, Inc. · US

Inventors

• Maksym A. Vodyanyk · Madison, US
• Xin ZHANG · Menlo Park, US
• Andrew J. BRANDL · Madison, US
• Deepika Rajesh · Madison, US
• Bradley Swanson · Madison, US
• Christie MUNN · Madison, US
• Sarah BURTON · Madison, US
• Wen Bo Wang · Waunakee, US

Key dates

• Filing date: Oct 20, 2016
• Grant date: Mar 16, 2021
• Expiry date: Oct 20, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/20
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.