Using proteases to control restriction enzyme activity
US10947518B1 · kind B1 · utility
3Cited by
1References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 2, 2020 |
| Grant date | Mar 16, 2021 |
| Priority date | — |
| Expiry date | Oct 2, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21062
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.