Mutated immunoglobulin-binding polypeptides

US10954309B2 · kind B2 · utility

Assignee

• Cytiva BioProcess R&D AB · SE

Inventors

• Gustav Rodrigo · Uppsala, SE
• Tomas Bjorkman · Uppsala, SE
• Mats Ander · Uppsala, SE

Key dates

• Filing date: Nov 11, 2015
• Grant date: Mar 23, 2021
• Expiry date: Nov 11, 2035

Classification

• Technology area (CPC —): General

Abstract

An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.