Preparation of factor Xa derivatives
US10954504B2 · kind B2 · utility
1Cited by
3References
14Claims
0Family size
Assignee
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Key dates
| Filing date | Jan 10, 2020 |
| Grant date | Mar 23, 2021 |
| Priority date | — |
| Expiry date | Jan 10, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods for manufacturing a fXa derivative protein at large scale leading to high yield of highly pure protein product. The method may include adding a detergent to a sample that contains a polynucleotide construct encoding the protein and purifying the protein through a soybean trypsin inhibitor (STI)-based affinity chromatograph, an ion exchange and mixed mode chromatograph and a hydrophobic interaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.