Assay system to identify HPV replication inhibitors in HT-screen
US10954545B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 2016 |
| Grant date | Mar 23, 2021 |
| Priority date | — |
| Expiry date | Mar 2, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A U2OS-based model system using luciferase reporters to monitor the genome replication of alpha and beta HPVs is provided. A modified U2OS cell line is disclosed that expresses Firefly luciferase to measure toxicity of the screened compounds. In addition, provided are HPV18, HPV 16 and HPV5 marker genomes that express Renilla luciferase under the control of viral promoters used to measure changes in the viral copy number. This ready-to-use model system is capable of being used in high-throughput screens to identify compounds inhibiting initial amplification and stable maintenance as well as vegetative phase of various HPV subtypes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.