Microfluidic devices and related methods for generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs
US10961492B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 28, 2016 |
| Grant date | Mar 30, 2021 |
| Priority date | — |
| Expiry date | Jan 24, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M35/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A microfluidic device for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs that includes a culture chamber may include: a confinement apparatus configured to define at least one compartment configured to contain a cellular matrix and at least one compartment configured to contain a culture medium, the confinement apparatus being hydrophobic and pervious to the culture medium; and/or at least one counter element. The confinement apparatus and at least one counter element may be reciprocally mobile between resting and compression positions of the cellular matrix. A method for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs at a microscale may include: controlled compression of a cellular matrix for a predetermined period of time. The cellular matrix may be delimited by a confinement apparatus that is pervious to a culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.