Generating mature lineages from induced pluripotent stem cells with MECP2 disruption

US10961505B2 · kind B2 · utility

Assignee

• FUJIFILM Cellular Dynamics, Inc. · US

Inventors

• Deepika Rajesh · Madison, US
• Anne STROUSE · Madison, US
• Sarah BURTON · Madison, US
• Christie MUNN · Madison, US
• Bradley Swanson · Madison, US

Key dates

• Filing date: Oct 5, 2017
• Grant date: Mar 30, 2021
• Expiry date: Oct 27, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2510/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.