Patent · US Active

Carbonyl reductase variant and its use in preparation of (R)-4-chloro-3-hydroxybutyrate

US10961515B1 · kind B1 · utility

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11Claims

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Assignee

FUDAN UNIVERSITY · CN

Inventors

Fener CHEN · Shanghai, CN
Zedu Huang · Shanghai, CN
Zexu Wang · Shanghai, CN
Minjie LIU · Shanghai, CN

Key dates

Filing date	Jun 17, 2020
Grant date	Mar 30, 2021
Priority date	—
Expiry date	Jun 17, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y101/01184
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein are a carbonyl reductase variant and its use in the preparation of (R)-4-chloro-3-hydroxybutyrate. The carbonyl reductase variant is obtained by mutating phenylalanine-85 in an amino acid sequence as shown in SEQ ID NO:4 to methionine. An amino acid or amino acids at one or more positions other than position 85 in the amino acid sequence of the carbonyl reductase may be further replaced. The application also provides a recombinant expression vector carrying the gene encoding the carbonyl reductase variant, a genetically-engineered bacterium carrying the carbonyl reductase variant gene and glucose dehydrogenase gene, and an application of this bacterium in the asymmetric reduction of 4-chloroacetoacetate to prepare (R)-4-chloro-3-hydroxybutyrate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.