Proline hydroxylase and uses thereof
US10961516B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 4, 2016 |
| Grant date | Mar 30, 2021 |
| Priority date | — |
| Expiry date | Nov 4, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/11002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or (c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.