Molecular probe for signal amplification and kit and assay using the same
US10962530B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 14, 2017 |
| Grant date | Mar 30, 2021 |
| Priority date | — |
| Expiry date | Feb 8, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/5412
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A molecular probe includes a detector molecule specific to a target molecule, and at least one label linked covalently or non-covalently to the detector molecule. The label includes a catalyst for generating electrons and/or mediators from a solution. The catalyst includes a nanomaterial, an enzyme, a metal, and/or a metal complex. The mediators can accumulate in the solution for a period of time. The detector molecule and the target molecule interact with each other by a protein based or nucleotide sequence based interaction. The mediators participate in a chemical reaction that generates a signal that may be a change in optical, electromagnetic, thermodynamic or mechanical properties. Generation efficiency of the mediators is enhanced by providing an energy to the solution. The molecular probe may be used with a molecular detection assay performed on a surface. A method for assaying the target molecule using the molecular probe is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.