Method for estimating the probe-target affinity of a DNA chip and method for manufacturing a DNA chip
US10964407B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 30, 2015 |
| Grant date | Mar 30, 2021 |
| Priority date | — |
| Expiry date | May 17, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B25/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method for estimating the affinity ϕ of a first DNA strand, or “probe”, to be hybridized with a second DNA strand, or “target”, to form a hybrid of length Lbp, the method comprising: in each division of a set of M divisions of the hybrid, counting the number of times in which each hybrid of a set of P DNA strand hybrids is present in the division, the hybrids being of length k less than the length Lbp, or “k-hybrids”; for each combination of mismatches of a set of L combinations of mismatches in a hybrid of length Lbp, determining whether the pair of mismatches is present in the hybrid; and calculating the affinity ϕ according to the relation:
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.