Method and system for taking measurements in a high-throughput screening with high time resolution

US10969337B2 · kind B2 · utility

Assignee

• Bayer Pharma Aktiengesellschaft · DE

Inventors

• Andreas Schade · Lengede, DE
• Mike Küster · Düsseldorf, DE
• Klaus Ochmann · Leverkusen, DE
• Michael Harnau · Leichlingen, DE
• Karl-Hermann Koeching · Dormagen, DE
• Nils Burkhardt · Velbert, DE
• Bernd Kalthof · Wuppertal, DE
• Linn Schneider · Düsseldorf, DE
• Georg Schmidt · Lindflur, DE

Key dates

• Filing date: Dec 14, 2017
• Grant date: Apr 6, 2021
• Expiry date: Dec 14, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6419
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and a system for rapid kinetic fluorescence measurement in high-throughput screening with high time-resolution includes providing a microtiter plate having a transparent base and multiple assay wells, a dispensing system arranged above the microtiter plate for simultaneously adding liquid into multiple assay wells, an illumination system beneath the microtiter plate for illuminating the multiple assay wells simultaneously through the transparent base, a detection system for detecting electromagnetic radiation from the multiple assay wells simultaneously through the transparent base, adding 0.3-300 μl of liquid per assay well from the dispensing system into the multiple assay wells, illuminating the multiple assay wells simultaneously through the transparent base using the illumination system before or from the time point of the start of the addition, detecting the electromagnetic radiation from the multiple assay wells simultaneously at a time interval of 1-1000 ms between individual measurement points.