Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

US10973229B2 · kind B2 · utility

Assignees

• DOW AGROSCIENCES LLC · US
• M.S. Technologies, L.L.C. · US

Inventors

• Yunxing C. Cui · Carmel, US
• Thomas Hoffman · Zionsville, US
• Ning Zhou · East Hanover, US
• Stephen Novak · Westfield, US
• Julissa Colon · Cidra, US
• Dawn M. Parkhurst · Westfield, US
• Sandra G. Toledo · Zionsville, US
• Terry R. Wright · Carmel, US
• Sean M. Russell · Carmel, US
• Bruce Held · Ames, US
• Vaithilingam Sekar · Ames, US

Key dates

• Filing date: Jun 7, 2019
• Grant date: Apr 13, 2021
• Expiry date: Sep 21, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.