De novo synthesized nucleic acid libraries
US10975372B2 · kind B2 · utility
17Cited by
581References
16Claims
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Key dates
| Filing date | Jul 10, 2018 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Jan 10, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.