Methods of utilizing thermostable mismatch endonuclease
US10975415B2 · kind B2 · utility
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Key dates
| Filing date | Sep 9, 2015 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Dec 12, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.