Rapid PCR-based virus detection method, and kits therefor
US10975418B1 · kind B1 · utility
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19Claims
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Key dates
| Filing date | Aug 4, 2020 |
| Grant date | Apr 13, 2021 |
| Priority date | — |
| Expiry date | Aug 4, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a rapid and high-sensitivity method to detect virus by qPCR nucleic acid amplification. A viral sample containing a RNA virus such as a corona virus is mixed with a virus preservation solution and a nucleic acid amplification reaction preparation (which can be in a lyophilized powder) to prepare the PCR-sample solution. The virus preservation solution can preserve the viral sample and prevent RNA degradation at room temperature for an extended period of time. No viral nucleic acid extraction step is needed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.